Call for tender for laboratories

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Call for partner laboratories

PEPPER is launching its fourth validation cycle.

We are looking for “partner labs “ ready to participate to the pre-validation of the selected methods.

It implies 2 steps :

  • a first step to assess method transferability (can partner labs successfully implement the methods with consistent results)
  • a second step to assess relevance and reliability with blind-coded substances

The final outcome of the project is to submit the pre-validated methods to international organisations (such as the OECD) to increase the regulatory tools available for the characterisation of endocrine disrupters.

A brief description of the methods is provided below to allow laboratories to assess if they have the necessary equipment and expertise.

Please contact us at philippe.hubert@ed-pepper.eu, or Andrea.Rivero-Arze@ed-pepper.eu

The laboratories who want to present an offer, can download the following documents presenting more detailed information:

  1. Last October,  the Method “Neurite outgrowth-Neuronal differentiation of C17.2 in response to RAR, RXR and PPARδ” was selected by the Pepper Project Opportunity Committee. This method, which has been developed during the ENDpoiNTs project at Uppsala university (Sweden), aims to identify endocrine disrupting properties impacting on neurodevelopment. In brief, this in vitro method, uses murine neuroprogenitor cells (C17.2) to study acute neurotoxicity and developmental neurotoxicity and effects on neurite morphology; by immunofluorescence microscopy via high-content imaging. It aims to determine whether a product acts via two endocrine pathways: retinoid and peroxisome proliferator-activated receptor delta (PPARδ). Deadline for offers on the Neurite method: May 30th, 2024.

Another call fot the “Fluorescent FITC-T4 Transthyretin Competitive Binding Assay for endocrine disruptors” method will be released soon. Stay tuned!